Journal: Journal of Inflammation Research

Article Title: Fire Needling Acupuncture Reduces Neutrophil Extracellular Traps (NETs) Formation and Inhibits TLR4/NF-κB/LCN2 Pathway in Psoriasis-Like Skin Lesions

doi: 10.2147/JIR.S569462

Figure Lengend Snippet: Fire needling acupuncture inhibits TLR4/MyD88/NF-κB/LCN2 pathway. ( A ) Representative Western blots showing TLR4, MyD88, NF-κB, p-NF-κB, and LCN2 in the epidermis of psoriasis-like skin lesions. ( B – E ) Comparison of TLR4, MyD88, p-NF-κB, and LCN2 protein levels between different groups (n = 3 for each group). ### P < 0.001 vs Control; * P < 0.05, ** P < 0.01 vs IMQ.

Article Snippet: After blocking nonspecific binding sites, primary antibodies against TLR4 (1:4000; Proteintech, USA), MyD88 (1:10000; Proteintech, USA), NF-κB (1:1000; Proteintech, USA), p-NF-κB (1:1000; CST, USA), LCN2 (1:1000; Proteintech, USA), and β-actin (1:1000; Proteintech, USA) were added, and PVDF membranes were incubated overnight at 4°C.

Techniques: Western Blot, Comparison, Control

Journal: Journal of Inflammation Research

Article Title: Fire Needling Acupuncture Reduces Neutrophil Extracellular Traps (NETs) Formation and Inhibits TLR4/NF-κB/LCN2 Pathway in Psoriasis-Like Skin Lesions

doi: 10.2147/JIR.S569462

Figure Lengend Snippet: Fire needling acupuncture reduces NETs formation and inhibits TLR4/MyD88/NF-κB/LCN2 pathway in psoriasis (the red arrows indicate inhibition).

Article Snippet: After blocking nonspecific binding sites, primary antibodies against TLR4 (1:4000; Proteintech, USA), MyD88 (1:10000; Proteintech, USA), NF-κB (1:1000; Proteintech, USA), p-NF-κB (1:1000; CST, USA), LCN2 (1:1000; Proteintech, USA), and β-actin (1:1000; Proteintech, USA) were added, and PVDF membranes were incubated overnight at 4°C.

Techniques: Inhibition

Journal: Scientific Reports

Article Title: 6-Gingerol alleviates high glucose-induced inflammation and cytotoxicity in THP-1 cells by inhibiting TLR4 signaling

doi: 10.1038/s41598-025-34192-z

Figure Lengend Snippet: 6-Gingerol attenuates high glucose-induced expression of Toll-like receptors in THP-1 cells. ( A ) Western blot analysis of TLR2 and TLR4 protein expression in THP-1 cells treated with glucose (0, 5.5 or 25 mM) and 6-gingerol (30 or 60 µM) for 24 h. ( B ) Quantitative real-time PCR (qPCR) analysis of TLR2 and TLR4 mRNA levels under the same treatment conditions. mRNA expression was normalized to GAPDH and expressed relative to control (set as 1.00). ( C ) Flow cytometric analysis of surface TLR2 and TLR4 expression. Cells were stained with fluorophore-conjugated anti-TLR2 and anti-TLR4 antibodies and analyzed using FACS. Data represent the mean ± SEM from three independent experiments. # p < 0.001 versus control; *** p < 0.001 by one-way ANOVA.

Article Snippet: Primary antibodies against TLR4 (sc-293072), p-IκBα (sc-8404), and β-actin (sc-47778), along with secondary antibodies (anti-mouse (sc-516102) and anti-rabbit (sc-2357)), COX‐1 (sc‐19998), COX‐2 (sc‐19999), cyclin E (sc-481), p21 (sc-756), and CDK4 (sc-260), were acquired from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA).

Techniques: Expressing, Western Blot, Real-time Polymerase Chain Reaction, Control, Staining

Journal: Poultry Science

Article Title: Music intervention mitigates LPS-induced gut barrier disruption and immune stress in broilers via TLR4/NF-κB regulation

doi: 10.1016/j.psj.2025.105189

Figure Lengend Snippet: Music has potential anti-inflammatory effects and can effectively alleviate inflammation in the jejunum and ileum by inhibiting the LPS-triggered TLR4/NF-κB signaling pathway. (A) The figure shows the relative mRNA expression levels of the TLR4/NF-κB inflammatory signaling pathway and related inflammatory factors in the jejunum and ileum of broilers. (B) The figure presents the immunoblot images and corresponding statistical results of TLR4, IKB-α, NF-κB p65, and TNF-α proteins in the jejunum and ileum of broilers. (C) The figure shows the measurement results of IL-1β, IL-6, and IL-10 levels in the jejunum and ileum of broilers across different groups. Differences marked with lowercase letters denote a significant difference ( P < 0.05), while the same letter indicates no significant difference ( P > 0.05).

Article Snippet: The membrane was then blocked in 5 % skim milk at 37°C for 2 h. After blocking, the membrane was incubated with diluted primary antibodies against TLR4, IKB-α, NF-κB, and TNF-α (1:1000, Abmart, China) at 4°C for 12 h. The specific antibodies used are listed in STable 6 .

Techniques: Expressing, Western Blot